8C87

Double mutant A(L172)C/L(L246)C structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	120
Detector technology	PIXEL
Collection date	2021-07-29
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 42 21 2
Unit cell lengths	99.725, 99.725, 239.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.450 - 2.450
R-factor	0.2611
Rwork	0.259
R-free	0.30820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.354
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.300	2.300
Rmeas	0.110	0.600
Number of reflections	53136	5315
<I/σ(I)>	8.26
Completeness [%]	97.3	82.7
Redundancy	6.9
CC(1/2)	0.960	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	1-mono-oleyl-rac-glycerol (MO), 1,2,3-heptanetriol, Jeffamine M600, N,N-dimethyldodecylamine-N-oxide (LDAO)