8C7T
Unliganded transcriptional pleiotropic repressor CodY from Enterococcus faecalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.988, 171.241, 215.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.870 - 2.210 |
R-factor | 0.2376 |
Rwork | 0.236 |
R-free | 0.27340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.291 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.870 | 2.290 |
High resolution limit [Å] | 2.210 | 2.210 |
Rpim | 0.077 | 0.876 |
Number of reflections | 62752 | 6079 |
<I/σ(I)> | 7.3 | 1.1 |
Completeness [%] | 99.9 | |
Redundancy | 6.4 | |
CC(1/2) | 0.995 | 0.501 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Protein, 600 microM in 20 mM TrisCl pH 8, 450 mM NaCl Well, 200 mM ammoniumhydrogenphosphate, 2.5% EtOH, 23-25% PEG3350 |