8C7G
Drosophila melanogaster Rab7 GEF complex Mon1-Ccz1-Bulli
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Energy Filter | TFS Selectris |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Software | cryoSPARC,PHENIX,EPU,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
