8C6V
The RNA silencing suppressor P8 from High-Plains Wheat Mosaic Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.960, 152.390, 65.400 |
Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
Resolution | 49.090 - 2.150 |
R-factor | 0.2015 |
Rwork | 0.200 |
R-free | 0.23360 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.469 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX (v1.20.1-4487-000) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 30.000 | 2.200 |
High resolution limit [Å] | 2.150 | 20.000 | 2.150 |
Rmerge | 0.073 | 0.050 | 1.914 |
Rmeas | 0.075 | 0.051 | 1.982 |
Number of reflections | 36581 | 37 | 2307 |
<I/σ(I)> | 24.45 | ||
Completeness [%] | 94.9 | ||
Redundancy | 20.1 | ||
CC(1/2) | 1.000 | 1.000 | 0.649 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 289 | 22% PEG 3350 0.1M Tris pH 7.5 0.2M NaCl 0.4M Guanidine HCL |