8C6E

CRYSTAL STRUCTURE OF ODORANT BINDING PROTEIN 4 FROM ANOPHELES GAMBIAE (AGAMOBP4) AT PH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-13
Detector	MAR CCD 165 mm
Wavelength(s)	1.03796
Spacegroup name	P 2 21 21
Unit cell lengths	33.950, 55.580, 56.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.540 - 2.050
R-factor	0.192
Rwork	0.190
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3N88
RMSD bond length	0.012
RMSD bond angle	1.301
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.540	2.160
High resolution limit [Å]	2.050	2.050
Rmeas	0.067	0.183
Rpim	0.025	0.067
Total number of observations		7131
Number of reflections	7086	1001
<I/σ(I)>	21.2	9.8
Completeness [%]	99.8
Redundancy	7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	20% W/V PEG 8000, 0.2 M MAGNESIUM CHLORIDE, 0.1 M TRIS-HCL PH 8.5,