8C6B
Light SFX structure of D.m(6-4)photolyase at 20ps time delay
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SwissFEL ARAMIS BEAMLINE ESA |
| Synchrotron site | SwissFEL ARAMIS |
| Beamline | ESA |
| Temperature [K] | 293.15 |
| Detector technology | PIXEL |
| Collection date | 2021-09-27 |
| Detector | PSI JUNGFRAU 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 |
| Unit cell lengths | 103.900, 103.900, 52.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.720 - 1.900 |
| R-factor | 0.2276 |
| Rwork | 0.227 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.886 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.720 | 1.910 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 44140 | 1766 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 275.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.5 | 277.15 | 100 mM bis-tris pH=6.5, 200 mM lithium sulphate monohydrate, 22 % PEG 3350, 0.5 % Ethyl acetate. |
