8C5X
Double mutant A(L37)C/S(L99)C structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-08 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.850, 139.850, 186.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.890 - 2.600 |
R-factor | 0.1868 |
Rwork | 0.186 |
R-free | 0.20740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.122 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.170 | 1.600 |
Number of reflections | 65303 | 4784 |
<I/σ(I)> | 10.13 | 1.42 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.6 | |
CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 3.5% 1,2,3 -heptanetriol, 2% dioxane, 0.1% LDAO, 1M potassium phosphate |