8C5I
Cyanide dihydratase from Bacillus pumilus C1 variant - Q86R,H305K,H308K,H323K
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.15 |
| Software | RELION,PHENIX,ISOLDE,Coot,UCSF Chimera |
| CTF correction | PHASE FLIPPING ONLY |
