8C5D
Glutathione transferase P1-1 from Mus musculus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.617, 77.366, 101.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.680 - 1.280 |
R-factor | 0.1756 |
Rwork | 0.175 |
R-free | 0.19990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.924 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.620 | 1.300 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmeas | 0.059 | 2.485 |
Number of reflections | 114851 | 5532 |
<I/σ(I)> | 12.7 | |
Completeness [%] | 99.7 | 98.3 |
Redundancy | 6.7 | 6.3 |
CC(1/2) | 0.990 | 0.350 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | PEG 6000 20% (w/v), 0.2 M calcium chloride dihydrate, pH 8.0 |