8C5C
microtubule decorated with tubulin oligomers in presence of APC C-terminal domain. (here only map corresponding to the 13-pf microtubule is represented)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 5.3 |
| Software | RELION,EPU,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
