8C5B

Crystal Structure of Penicillin-binding Protein 3 (PBP3) from Staphylococcus Epidermidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	83.442, 83.442, 307.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.940 - 2.500
R-factor	0.2229
Rwork	0.221
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.049
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (v1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.687	2.297
High resolution limit [Å]	2.067	2.067
Number of reflections	46353	2318
<I/σ(I)>	16.8
Completeness [%]	95.2
Redundancy	18.8
CC(1/2)	0.999	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Ammonium sulfate