8C56
CpG specific M.MpeI methyltransferase crystallized in the presence of 2'-deoxy-5-methylzebularine (5mZ) and 5-methylcytosine containing dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 84.259, 84.259, 173.883 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 2.400 |
R-factor | 0.17535 |
Rwork | 0.173 |
R-free | 0.21625 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dkj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.006 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.121 | 1.277 |
Number of reflections | 25251 | 3914 |
<I/σ(I)> | 16.89 | 2.05 |
Completeness [%] | 99.6 | 99 |
Redundancy | 8.6 | 8.8 |
CC(1/2) | 0.999 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 10% PEG 3350, 150 mM NaCl, 50 mM sodium citrate (final pH, 5.6). For cryoprotection glycerol was added to 25% v/v |