8C4D

N-acetylmuramoyl-L-alanine amidase from Enterococcus faecium prophage genome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 43 2 2
Unit cell lengths	74.713, 74.713, 133.272
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.730 - 1.970
R-factor	0.1749
Rwork	0.173
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.878
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.730	2.030
High resolution limit [Å]	1.970	1.970
Rmeas	0.460	4.630
Number of reflections	27661	2614
<I/σ(I)>	9
Completeness [%]	99.9	99.1
Redundancy	25.5
CC(1/2)	0.980	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	20% w/v PEG 3000, 0.1 M sodium citrate, pH 5.5