8C3H
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex a long aspartimide degron peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-03 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.256, 58.821, 88.858 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.530 - 1.710 |
| Rwork | 0.179 |
| R-free | 0.21040 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.769 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 1.810 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.082 | 1.629 |
| Number of reflections | 32554 | 5215 |
| <I/σ(I)> | 14.3 | 1.15 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 12.84 | 12.42 |
| CC(1/2) | 1.000 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 274 | 0.5 M (NH4)H2PO4 |
