8C37
An intermediate light exposed 2.15 Angstrom crystal structure of H132A variant of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under anaerobic conditions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-09 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.821, 69.683, 201.809 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.180 - 2.150 |
R-factor | 0.19528 |
Rwork | 0.193 |
R-free | 0.22967 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8c35 |
RMSD bond length | 0.008 |
RMSD bond angle | 2.107 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.820 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 48484 | 2376 |
<I/σ(I)> | 18.2 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.4 | 13.7 |
CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 0.1 M ammonium sulfate, 0.1 M sodium citrate pH 5.8, 16% w/v PEG 4000, 20% v/v glycerol |