8C36
Light-adapted 2.0 Angstrom crystal structure of H132A variant of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under aerobic conditions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.405, 70.664, 203.687 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.850 - 2.000 |
R-factor | 0.18293 |
Rwork | 0.181 |
R-free | 0.21830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8c35 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.933 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.900 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 62906 | 4599 |
<I/σ(I)> | 10.3 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.5 | 13.5 |
CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 0.1 M ammonium sulfate, 0.1 M sodium citrate pH 5.8, 16% w/v PEG 4000, 20% v/v glycerol |