8C27
Domain 3 of Group B Streptococcus pilus protein as scaffold for the display of foreign epitopes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 71.391, 118.047, 189.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.150 - 2.630 |
| R-factor | 0.2292 |
| Rwork | 0.227 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xtl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.822 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.900 | 94.900 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 12062 | 12062 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.8 | |
| CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES with 20% w/v jeff ED-2001 pH 6.5 |
