8C1X
sfGFP C148 F206 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-30 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.81532 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 67.489, 73.763, 127.277 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 39.240 - 1.890 |
| R-factor | 0.20843 |
| Rwork | 0.206 |
| R-free | 0.24703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.806 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.250 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 100181 | 4910 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.999 | 0.225 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Sodium malonate dibasic monohydrate, 0.1 M Bis-Tris propane, pH 7.5, 20 % w/v PEG 3350 |
