8C1J

Crystal Structure of Mus musculus Protein Arginine Methyltransferase 2 in complex with RSF1_18-30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	66.178, 114.964, 133.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.690 - 2.000
R-factor	0.181
Rwork	0.180
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	0.996
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.690	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.099	3.410
Rmeas	0.101	3.488
Rpim	0.019	0.708
Number of reflections	34584	2361
<I/σ(I)>	19.4	1
Completeness [%]	99.6	94.4
Redundancy	26.7	23.1
CC(1/2)	1.000	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.1M ammonium tartrate