8C0G

SARS-CoV nsp16-nsp10 complexed with N7-GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-21
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97930
Spacegroup name	C 2 2 21
Unit cell lengths	67.232, 185.042, 128.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.520 - 1.880
R-factor	0.2457
Rwork	0.245
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	XDS (20220220)
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	92.520	92.520	1.910
High resolution limit [Å]	1.880	5.097	1.880
Rmerge	0.180	0.120	3.680
Rmeas	0.194	0.130	4.120
Rpim	0.072	0.050	1.790
Number of reflections	472780	3494	3217
<I/σ(I)>	3.6	7.1	0.5
Completeness [%]	100.0	99.7	99.7
Redundancy	7.2	7	5
CC(1/2)	0.988	0.983	0.322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	67 MM M CHES, 0.99 M MGCL2-HEXAYDRATE, 33 MM TRIS-HCL, 8.3% (V/V) PEG 8000