8C0D

UFL1/DDRGK1 bound to UFC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-09
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.873128
Spacegroup name	P 21 21 21
Unit cell lengths	79.210, 124.565, 131.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.732 - 2.564
Rwork	0.232
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.888
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.732	2.892
High resolution limit [Å]	2.564	2.564
Number of reflections	25858	1293
<I/σ(I)>	8.2
Completeness [%]	93.8
Redundancy	12.8
CC(1/2)	0.997	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	292	0.1M HEPES, 1.03M Li2SO4