8BZM
FOXK1-ELF1-heterodimer bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.6199 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.181, 105.868, 68.194 |
Unit cell angles | 90.00, 112.94, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.690 |
R-factor | 0.27352 |
Rwork | 0.271 |
R-free | 0.32360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.693 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.490 | 2.840 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.200 | 1.999 |
Rmeas | 0.232 | 2.310 |
Rpim | 0.116 | 1.147 |
Total number of observations | 92854 | 13710 |
Number of reflections | 23997 | 3493 |
<I/σ(I)> | 3.9 | 0.4 |
Completeness [%] | 99.8 | |
Redundancy | 3.9 | 3.9 |
CC(1/2) | 0.991 | 0.399 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | PEG MME 3350, PEG 200, magnesium chloride, HEPES |