8BZK

Crystal structure of Paradendryphiella salina PL7C alginate lyase with sulphate bound in the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	38.420, 56.530, 87.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.190 - 1.450
R-factor	0.1506
Rwork	0.150
R-free	0.17410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.976
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.510	1.540
High resolution limit [Å]	1.450	1.450
Number of reflections	65393	10605
<I/σ(I)>	7.76
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	1.000	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1M NaOAc pH 4.6, 2M (NH4)2SO4