8BZC

co-soaked stabilizers for ERa - 14-3-3 interaction (884_AZ244)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.033200
Spacegroup name	C 2 2 21
Unit cell lengths	81.723, 112.327, 62.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.360 - 1.100
R-factor	0.1718
Rwork	0.172
R-free	0.17680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.080	1.120
High resolution limit [Å]	1.100	1.100
Number of reflections	1083665	667
<I/σ(I)>	20.8
Completeness [%]	83.0
Redundancy	11.3
CC(1/2)	0.999	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.095 M HEPES (pH 7.1), PEG400 (29% (v/v)), 0.19 M CaCl2 and 5% (v/v) Glycerol