8BZ7

Crystal structure of the L. monocytogenes RmlT in complex with TDP-rhamnose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979257
Spacegroup name	P 1 21 1
Unit cell lengths	86.988, 291.265, 92.394
Unit cell angles	90.00, 100.38, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.1887
Rwork	0.187
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8bz4
RMSD bond length	0.005
RMSD bond angle	0.670
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.059	1.087
Rmeas	0.069	1.275
Rpim	0.035	0.658
Number of reflections	227206	11233
<I/σ(I)>	14	1.2
Completeness [%]	99.8
Redundancy	3.8
CC(1/2)	0.999	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis-Tris propane pH 7.0 0.2 M Potassium sodium tartrate tetrahydrate 20% (w/v) PEG 3,350