8BZ6
Crystal structure of the L. monocytogenes RmlT in complex with UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978565 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.523, 291.057, 89.824 |
Unit cell angles | 90.00, 100.08, 90.00 |
Refinement procedure
Resolution | 44.220 - 2.400 |
R-factor | 0.1968 |
Rwork | 0.195 |
R-free | 0.23510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8bz4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.581 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.340 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.103 | 1.560 |
Rmeas | 0.111 | 1.678 |
Rpim | 0.041 | 0.615 |
Number of reflections | 166303 | 8154 |
<I/σ(I)> | 12.2 | 1.3 |
Completeness [%] | 99.1 | 97.7 |
Redundancy | 7.2 | 7.3 |
CC(1/2) | 0.998 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl pH 8.5 0.2 M Sodium acetate 15% (w/v) PEG 4,000 |