8BZ4

Crystal structure of the apo form of the L. monocytogenes RmlT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 21
Unit cell lengths	83.427, 103.688, 178.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.780 - 2.520
R-factor	0.2206
Rwork	0.219
R-free	0.25140
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.521
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.780	2.600
High resolution limit [Å]	2.520	2.520
Rmerge	0.210	1.735
Rmeas	0.218	1.804
Rpim	0.059	0.491
Number of reflections	53098	4546
<I/σ(I)>	9.8	1.5
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	0.997	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Succinic acid pH 7.0 10% (w/v) PEG 3,350