8BZ2
Crystal structure of outer membrane attachment porin OmpM1 SLH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-29 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 0.8984 |
Spacegroup name | H 3 |
Unit cell lengths | 118.710, 118.710, 47.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.780 - 1.700 |
R-factor | 0.225 |
Rwork | 0.224 |
R-free | 0.24900 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.011 |
RMSD bond angle | 1.219 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.780 | 1.600 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.049 | 1.720 |
Rpim | 0.017 | 0.884 |
Number of reflections | 34489 | 1590 |
<I/σ(I)> | 17.1 | 0.7 |
Completeness [%] | 99.5 | 92.9 |
Redundancy | 9 | 4.7 |
CC(1/2) | 1.000 | 0.230 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 0.1 M citric acid pH 3.5 2.0 M ammonium sulphate |