8BYX

HOXB13-homodimer bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	P 43 21 2
Unit cell lengths	116.779, 116.779, 118.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.000
R-factor	0.25635
Rwork	0.254
R-free	0.30432
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.408
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.030	3.070
High resolution limit [Å]	2.910	2.910
Rmeas	0.134	4.116
Rpim	0.050	1.458
Total number of observations	138874	19863
Number of reflections	18396	2551
<I/σ(I)>	8.6	0.7
Completeness [%]	99.5
Redundancy	7.5	7.8
CC(1/2)	0.995	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	24 % PEG 1500 MME, 0.15M potassium chloride, 0.05M Tris-HCl buffer, 2%PEG 400