8BXQ

fragment-linked stabilizer for ERa - 14-3-3 interaction (1075296)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.033
Spacegroup name	C 2 2 21
Unit cell lengths	81.939, 111.903, 62.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.720 - 1.600
R-factor	0.1616
Rwork	0.161
R-free	0.18220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.032
Data reduction software	DIALS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.720	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	37311	1805
<I/σ(I)>	30.3
Completeness [%]	97.4
Redundancy	13
CC(1/2)	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.095 M HEPES (pH 7.1), PEG400 (29% (v/v)), 0.19 M CaCl2 and 5% (v/v) Glycerol