8BXP
SfGFP C148 F206 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-28 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.81532 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 67.804, 75.016, 125.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.300 - 1.790 |
| R-factor | 0.2018 |
| Rwork | 0.200 |
| R-free | 0.23442 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.795 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.360 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 61413 | 2999 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.5 | |
| CC(1/2) | 0.999 | 0.351 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium acetate trihydrate, 0.1 M Bis-tris propane, pH 7.5, 20% w/v PEG 3350 |
