8BXE
Alvinella pompejana nicotinic acetylcholine receptor Alpo4 in apo state (Alpo4_comb dataset 3)
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | In-column Omega Filter |
| Phase Plate | OTHER |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.9 |
| Software | crYOLO,cryoSPARC,PHENIX |
| Symmetry | C5 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
