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8BX1

Oct4/Sox2 protein:DNA complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron sitePETRA III, EMBL c/o DESY
BeamlineP13 (MX1)
Temperature [K]100
Detector technologyPIXEL
Collection date2021-06-08
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.97624
Spacegroup nameP 21 21 21
Unit cell lengths51.199, 65.892, 126.920
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution58.480 - 2.500
R-factor0.25414
Rwork0.253
R-free0.27410
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6ht5
RMSD bond length0.012
RMSD bond angle2.375
Data reduction softwareXDS
Data scaling softwareAimless (0.7.4)
Phasing softwareMOLREP (11.7.03)
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]126.9202.600
High resolution limit [Å]2.5002.500
Rmerge0.0911.280
Number of reflections154801711
<I/σ(I)>16.52
Completeness [%]99.7
Redundancy11.4
CC(1/2)0.9990.755
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP4.62770.1M sodium acetate, 12% (v/v) PEG 4000

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