8BX1
Oct4/Sox2 protein:DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97624 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.199, 65.892, 126.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.480 - 2.500 |
R-factor | 0.25414 |
Rwork | 0.253 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ht5 |
RMSD bond length | 0.012 |
RMSD bond angle | 2.375 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP (11.7.03) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.920 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.091 | 1.280 |
Number of reflections | 15480 | 1711 |
<I/σ(I)> | 16.5 | 2 |
Completeness [%] | 99.7 | |
Redundancy | 11.4 | |
CC(1/2) | 0.999 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.1M sodium acetate, 12% (v/v) PEG 4000 |