8BWK

Metagenomic derived PL6 alginate lyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	72.749, 228.850, 113.733
Unit cell angles	90.00, 101.86, 90.00

Refinement procedure

Resolution	63.860 - 2.290
R-factor	0.1817
Rwork	0.180
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.506
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.304	2.330
High resolution limit [Å]	2.290	2.290
Number of reflections	160343	6980
<I/σ(I)>	11.2
Completeness [%]	98.0
Redundancy	6.7
CC(1/2)	0.990	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	0.1 M Sodium citrate pH 5.8, 0.1 M (NH4)2SO4, 20% v/v Glycerol, and 16% w/v PEG 4000