8BWC
E. coli BAM complex (BamABCDE) wild-type
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | TFS Selectris |
Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Number of Particle | 656080 |
Software | crYOLO,PHENIX,ISOLDE,EPU,CTFFIND,RELION |
CTF correction | NONE |