8BVL

Crystal structure of the IBR-RING2 domain of HOIL-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2829
Spacegroup name	P 1 21 1
Unit cell lengths	53.908, 59.263, 57.206
Unit cell angles	90.00, 90.31, 90.00

Refinement procedure

Resolution	41.160 - 2.240
R-factor	0.2371
Rwork	0.234
R-free	0.29610
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.007
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.160	2.320
High resolution limit [Å]	2.240	2.240
Rmerge	0.080	0.218
Number of reflections	16782	1651
<I/σ(I)>	10.14	3.75
Completeness [%]	95.8
Redundancy	2.8
CC(1/2)	0.989	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	200 mM magnesium chloride hexahydrate 100mM Tris-HCl pH 7.0 10% PEG 8000