8BVG

Bright fluorescent protein BrUSLEE with subnanosecond fluorescence lifetime

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	75.659, 122.709, 167.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.720 - 2.380
R-factor	0.2041
Rwork	0.203
R-free	0.27110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EGFP
RMSD bond length	0.011
RMSD bond angle	1.404
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.490
High resolution limit [Å]	2.380	5.160	2.400
Rmerge	0.094	0.056	0.930
Rmeas	0.101	0.061	1.025
Rpim	0.038	0.023	0.424
Number of reflections	62234	6555	6107
<I/σ(I)>	8.9
Completeness [%]	99.9	100	99.3
Redundancy	6.9	6.9	5.6
CC(1/2)		0.998	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	6.4% Tacsimate pH 5.0, 16% PEG 3350