8BVA

Crystal Structure of Mus musculus Protein Arginine Methyltransferase 2 in complex with RSF1_114-126

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 2 2 21
Unit cell lengths	65.711, 114.409, 133.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.550 - 2.190
R-factor	0.1783
Rwork	0.177
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ful
RMSD bond length	0.006
RMSD bond angle	0.727
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.20.1)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.550	2.260
High resolution limit [Å]	2.190	2.190
Rmerge	0.106	1.480
Rmeas	0.110	1.541
Number of reflections	25946	2056
<I/σ(I)>	14.9	1.5
Completeness [%]	99.1	92.4
Redundancy	13.8	12.3
CC(1/2)	0.999	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2M ammonium tartrate