8BV8

Crystal structure of the phage Mu protein Mom inactive mutant S124A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033200
Spacegroup name	P 21 21 21
Unit cell lengths	57.180, 66.480, 138.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.940 - 2.030
R-factor	0.2162
Rwork	0.215
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.430
Data reduction software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.940	2.103
High resolution limit [Å]	2.030	2.030
Rmerge	0.186
Rmeas	0.194
Rpim	0.052
Number of reflections	34815	3412
<I/σ(I)>	14.64
Completeness [%]	99.7	99.5
Redundancy	13.4	14
CC(1/2)	0.999	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289.15	PEG 3350, potassium thiocyanate, bis-tris propane