8BV7

Crystal structure of the Trichoplax Scribble PDZ1 domain in complex with the Trichoplax phosphorylated Vangl peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	30.092, 45.236, 70.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.440 - 1.800
R-factor	0.2181
Rwork	0.217
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.611
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.240	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.095
Number of reflections	9419	541
<I/σ(I)>	13.3
Completeness [%]	99.4
Redundancy	10.9
CC(1/2)	0.999	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M Sodium bromide,0.1 M Bis-Tris propane pH 8.5, 20 % w/v PEG 3350, 20% Glucose