8BV1
Peptide inhibitor P4 in complex with ASF1 histone chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 43 |
| Unit cell lengths | 123.885, 123.885, 179.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.790 - 2.834 |
| R-factor | 0.2045 |
| Rwork | 0.204 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6f0h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.372 | 2.907 |
| High resolution limit [Å] | 2.834 | 2.834 |
| Rmerge | 0.156 | |
| Rmeas | 0.162 | |
| Rpim | 0.043 | 0.635 |
| Number of reflections | 56883 | 1058 |
| <I/σ(I)> | 13.13 | 1.446 |
| Completeness [%] | 88.8 | 22.49 |
| Redundancy | 14.27 | 13.97 |
| CC(1/2) | 0.998 | 0.607 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290.15 | 0.1 M Tris pH 8.5 30% (w/v) PEG 400 |
