8BV1

Peptide inhibitor P4 in complex with ASF1 histone chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 43
Unit cell lengths	123.885, 123.885, 179.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.790 - 2.834
R-factor	0.2045
Rwork	0.204
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f0h
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.372	2.907
High resolution limit [Å]	2.834	2.834
Rmerge	0.156
Rmeas	0.162
Rpim	0.043	0.635
Number of reflections	56883	1058
<I/σ(I)>	13.13	1.446
Completeness [%]	88.8	22.49
Redundancy	14.27	13.97
CC(1/2)	0.998	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290.15	0.1 M Tris pH 8.5 30% (w/v) PEG 400