8BV0

Binary complex between the NB-ARC domain from the Tomato immune receptor NRC1 and the SPRY domain-containing effector SS15 from the potato cyst nematode

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-06
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 61
Unit cell lengths	128.560, 128.560, 170.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.340 - 4.500
R-factor	0.2409
Rwork	0.237
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.794
Data reduction software	XDS
Data scaling software	Aimless (0.7.8)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.340	51.340	5.030
High resolution limit [Å]	4.500	10.060	4.500
Rmerge	0.084	0.055	1.513
Rmeas	0.088	0.057	1.651
Rpim	0.026	0.016	0.642
Total number of observations	77102	11573	13865
Number of reflections	8981	871	2263
<I/σ(I)>	7.2	20.6	1.5
Completeness [%]	94.0	99.1	84
Redundancy	8.6	13.3	6.1
CC(1/2)	0.998	0.997	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	NULL