8BV0
Binary complex between the NB-ARC domain from the Tomato immune receptor NRC1 and the SPRY domain-containing effector SS15 from the potato cyst nematode
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-06 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 61 |
| Unit cell lengths | 128.560, 128.560, 170.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.340 - 4.500 |
| R-factor | 0.2409 |
| Rwork | 0.237 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.794 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.8) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.340 | 51.340 | 5.030 |
| High resolution limit [Å] | 4.500 | 10.060 | 4.500 |
| Rmerge | 0.084 | 0.055 | 1.513 |
| Rmeas | 0.088 | 0.057 | 1.651 |
| Rpim | 0.026 | 0.016 | 0.642 |
| Total number of observations | 77102 | 11573 | 13865 |
| Number of reflections | 8981 | 871 | 2263 |
| <I/σ(I)> | 7.2 | 20.6 | 1.5 |
| Completeness [%] | 94.0 | 99.1 | 84 |
| Redundancy | 8.6 | 13.3 | 6.1 |
| CC(1/2) | 0.998 | 0.997 | 0.588 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | NULL |
