8BUW
Crystal structure of Trichoplax Scribble PDZ1 domain in complex with Trichoplax Vangl peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95372 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.547, 30.298, 89.460 |
Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
Resolution | 29.820 - 2.850 |
R-factor | 0.2292 |
Rwork | 0.227 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vwc |
RMSD bond length | 0.003 |
RMSD bond angle | 0.614 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.300 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 4011 | 587 |
<I/σ(I)> | 4.3 | |
Completeness [%] | 96.4 | |
Redundancy | 2.7 | |
CC(1/2) | 0.954 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M sodium acetate trihydrate, 0.1 M Tris pH 8.5, 30% PEG 4000, 30% (v/v) ethylene glycol |