8BUQ
Structure of DDB1 bound to DS43-engaged CDK12-cyclin K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-19 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 250.162, 250.162, 218.849 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.300 - 3.200 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 216.647 | 3.393 |
| High resolution limit [Å] | 3.199 | 3.199 |
| Number of reflections | 105078 | 5252 |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 96.4 | |
| Redundancy | 21 | |
| CC(1/2) | 0.999 | 0.463 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.9 M ammonium citrate tribasic pH 7.0, Jeff600 additive |
