8BUL

Structure of DDB1 bound to DS11-engaged CDK12-cyclin K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	249.356, 249.356, 217.993
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.270 - 3.400
R-factor	0.1792
Rwork	0.177
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.981
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	215.949	3.577
High resolution limit [Å]	3.400	3.402
Number of reflections	91223	4564
<I/σ(I)>	11.5
Completeness [%]	94.4
Redundancy	20.9
CC(1/2)	0.999	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.30 M amonium sulphate, 0.07 M HEPES, pH 6.8