8BUK

Structure of DDB1 bound to DS08-engaged CDK12-cyclin K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	248.824, 248.824, 217.984
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.660 - 3.410
R-factor	0.1862
Rwork	0.185
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.784
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	215.488	3.562
High resolution limit [Å]	3.406	3.406
Number of reflections	92889	4644
<I/σ(I)>	15.5
Completeness [%]	91.2
Redundancy	20.8
CC(1/2)	0.999	0.514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.84 M ammonium sulfate, 0.36 M ammonium citrate, 0.07 M HEPES pH 7