8BUJ

Structure of DDB1 bound to DS06-engaged CDK12-cyclin K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.99995
Spacegroup name	P 31 2 1
Unit cell lengths	249.353, 249.353, 218.417
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.780 - 3.620
Rwork	0.164
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.850
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	215.946	3.784
High resolution limit [Å]	3.618	3.618
Number of reflections	81004	4053
<I/σ(I)>	11.6
Completeness [%]	95.8
Redundancy	21.1
CC(1/2)	0.990	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.38 M ammonium sulphate, 0.07 M HEPES, pH 7.0