8BUJ
Structure of DDB1 bound to DS06-engaged CDK12-cyclin K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-20 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.99995 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 249.353, 249.353, 218.417 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 76.780 - 3.620 |
Rwork | 0.164 |
R-free | 0.20590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.850 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 215.946 | 3.784 |
High resolution limit [Å] | 3.618 | 3.618 |
Number of reflections | 81004 | 4053 |
<I/σ(I)> | 11.6 | |
Completeness [%] | 95.8 | |
Redundancy | 21.1 | |
CC(1/2) | 0.990 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.38 M ammonium sulphate, 0.07 M HEPES, pH 7.0 |