8BUI

Structure of DDB1 bound to DRF-053-engaged CDK12-cyclin K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	249.436, 249.436, 218.342
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.110 - 3.500
R-factor	0.1838
Rwork	0.182
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	216.018	3.697
High resolution limit [Å]	3.500	3.505
Number of reflections	84921	4246
<I/σ(I)>	14.7
Completeness [%]	96.1
Redundancy	21
CC(1/2)	0.999	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.42 M ammonium sulphate, 0.07 M HEPES, pH 6.8