8BU8

Double-motif Single-stranded Paranemic Crossover RNA Triangle (2PXT)

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	6.56
Software	cryoSPARC,EPU
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION