8BU7

Structure of DDB1 bound to 21195-engaged CDK12-cyclin K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	248.683, 248.683, 218.597
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.870 - 3.245
R-factor	0.1934
Rwork	0.192
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.815
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	215.366	3.396
High resolution limit [Å]	3.245	3.245
Number of reflections	110959	5549
<I/σ(I)>	16.4
Completeness [%]	96.1
Redundancy	21
CC(1/2)	0.999	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.33 M ammonium sulfate, 0.045 M ammonium citrate, 0.07M HEPES pH 7